Application of principal component analysis to determine the key structural features contributing to iron superoxide dismutase thermostability

作者：Ding, Yanrui^*; Cai, Yujie; Han, Yonggang; Zhao, Bingqiang; Zhu, Lei

来源：Biopolymers, 2012, 97(11): 864-872.

DOI：10.1002/bip.22093

• iron superoxide dismutase
• principal component analysis
• thermostability
• structural parameters

Iron superoxide dismutase (Fe-SOD) is predominantly found in bacteria and mitochondria. The thermal stability of Fe-SOD from different sources can vary dramatically. We have studied the influence of structural parameters on Fe-SOD thermostability by principal component analysis (PCA). The results show that an increased a-helical and turn content, an increased a-helix and loop length, an increase in the number of main-main chains and charged-uncharged hydrogen bonds, a decrease in the 310-helix content, and a decreased beta-strand and loop length are all important factors for Fe-SOD thermostability. Interestingly, the use of charged residues to form salt bridges is tendentious in thermophilic Fe-SOD. Negatively charged Arg and positively charged Glu are efficiently used to form salt bridges. The cooperative action of the exposed area, the hydrogen bonds, and the secondary structure plays a crucial role in resisting high temperatures, which demonstrates that the increased stability of thermophilic Fe-SOD is provided by several structural factors acting together.

• 出版日期2012-11
• 单位江南大学