Dynamic T cell-APC interactions sustain chronic inflammation in atherosclerosis

作者：Koltsova Ekaterina K; Garcia Zacarias; Chodaczek Grzegorz; Landau Michael; McArdle Sara; Scott Spencer R; von Vietinghoff Sibylle; Galkina Elena; Miller Yury I; Acton Scott T; Ley Klaus^*

来源：Journal of Clinical Investigation, 2012, 122(9): 3114-3126.

DOI：10.1172/JCI61758

Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries characterized by leukocyte accumulation in the vessel wall. Both innate and adaptive immune responses contribute to atherogenesis, but the identity of atherosclerosis-relevant antigens and the role of antigen presentation in this disease remain poorly characterized. We developed live-cell imaging of explanted aortas to compare the behavior and role of APCs in normal and atherosclerotic mice. We found that CD4(+) T cells were capable of interacting with fluorescently labeled (CD11c-YFP+) APCs in the aortic wall in the presence, but not the absence, of cognate antigen. In atherosclerosis-prone Apoe(-/-)CD11c-YFP+ mice, APCs extensively interacted with CD4(+) T cells in the aorta, leading to cell activation and proliferation as well as secretion of IFN-gamma and TNF-alpha. These cytokines enhanced uptake of oxidized and minimally modified LDL by macrophages. We conclude that antigen presentation by APCs to CD4(+) T cells in the arterial wall causes local T cell activation and production of proinflammatory cytokines, which promote atherosclerosis by maintaining chronic inflammation and inducing foam cell formation.

• 出版日期2012-9