Substitutional Analysis of the C-Terminal Domain of AbrB Revealed Its Essential Role in DNA-Binding Activity

作者：Neubauer Svetlana; Dolgova Olga; Praeg Gregory; Borriss Rainer; Makarewicz Oliwia^*

来源：PLos One, 2014, 9(5): e97254.

DOI：10.1371/journal.pone.0097254

The global transition state regulator AbrB controls more than 100 genes of the Bacillus relatives and is known to interact with varying DNA-sequences. The DNA-binding domain of the AbrB-like proteins was proposed to be located exclusively within the amino-terminal ends. However, the recognition of DNA, and specificity of the binding mechanism, remains elusive still in view of highly differing recognition sites. Here we present a substitutional analysis to examine the role of the carboxy-terminal domain of AbrB from Bacillus subtilis and Bacillus amyloliquefaciens. Our results demonstrate that the carboxy-terminal domains of AbrB affect the DNA-binding properties of the tetrameric AbrB. Most likely, the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA targets like tycA and phyC.

• 出版日期2014-5-15