Distinct expression patterns of two Ginkgo biloba 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase/isopentenyl diphospahte synthase (HDR/IDS) promoters in Arabidopsis model

作者：Kang Min Kyoung; Nargis Sultana; Kim Sang Min; Kim Soo Un^*

来源：Plant Physiology and Biochemistry, 2013, 62: 47-53.

DOI：10.1016/j.plaphy.2012.10.011

• 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase/isopentenyl diphosphate synthase (HDR/IDS)
• Isoprenoid
• beta-Galactosidase (GUS)
• Ginkgo biloba
• Ginkgolides
• Promoter

1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) or isopentenyl diphosphate synthase (IDS) is an enzyme at the final step of the MEP pathway. The multi-copy nature of IDS gene in a gymnosperm Ginkgo biloba is known. To evaluate the function of each isogene, the roles of the promoters were examined in Arabidopsis model. Among the promoters of GbIDS series, about 1.3 kb of GbIDS1pro and 1.5 kb of GbIDS2pro were cloned and fused with GUS. The GbIDS1pro::GUS was introduced into Arabidopsis to show GUS expression in most organs except for roots, petals, and stamina, whereas the GbIDS2pro::GUS was expressed only in the young leaves, internodes where the flower and shoot branched, and notably in primary root junction. This pattern of GUS expression correlated with high transcript level of GbIDS2 compared to that of GbIDS1 in Ginkgo roots. Methyl jasmonate (MeJA) treatment resulted in down-regulated GbIDS1pro activity in Arabidopsis leaves and upregulated GbIDS2pro activity in roots. The same pattern of gene regulation in roots was also seen upon treatments of gibberellins, abscisic acid, and indole butyric acid.

• 出版日期2013-1