GRP78 Participates in PCA3-regulated Prostate Cancer Progression

作者：Lin, Chunhua; Wang, Jiahui; Wang, Yanwei; Zhu, Peng; Liu, Xin; Li, Ning; Liu, Juan; Yu, Luxin^*; Wang, Wenting^*

来源：Anticancer Research, 2017, 37(8): 4303-4310.

DOI：10.21873/anticanres.11823

• Prostate cancer
• PCA3
• GRP78
• long non-coding RNA
• cancer-related genes

Background/Aim: The human prostate cancer antigen 3 (PCA3) is a long non-coding RNA (lncRNA) commonly used as a diagnostic marker for prostate cancer (PCa). Herein we investigated the cellular function of PCA3 in PCa and its potential mechanism. Materials and Methods: PCA3 was overexpressed in a PC3 cell line (PC3(PCA+)) and cell proliferation, migration, invasion and apoptosis were compared to those of control cells (PC3(NC)). Differentially expressed proteins were identified by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry. Results: Overexpression of PCA3 significantly increased cell proliferation rate, migration and invasion, while inhibited apoptosis in PC3 cells. Three proteins were found down-regulated and 7 proteins up-regulated in PC3(PCA+) cells compared to PC3(NC) cells, including GRP78. Higher GRP78 was also found in PCa clinical specimens. Conclusion: This study confirmed that in PCa, PCA3 plays a pro-cancer role through promoting cell proliferation, migration and invasion while inhibiting cell apoptosis. This process might involve the up-regulation of GRP78.

• 出版日期2017-8
• 单位烟台毓璜顶医院; 青岛大学