Activation of ERK1/2 and TNF-alpha production are regulated by calcium/calmodulin signaling pathway during Penicillium marneffei infection within humand macrophages

作者：Chen Renqiong; Ji Guangquan; Wang Ling; Ren Hong; Xi Liyan^*

来源：Microbial Pathogenesis, 2016, 93: 95-99.

DOI：10.1016/j.micpath.2016.01.026

• Calcium
• Calmodulin
• Macrophages
• Penicillium marneffei
• TNF-alpha

Previous study have shown that Penicillium marneffei (P. marneffei)-induced TNF-alpha production via an extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase-dependent mechanism is an important host defence mechanism against P. marneffei in human macrophages. Therefore, we explore signaling pathway that regulates TNF-alpha secretion and activation of ERK1/2 by intracellular signaling mechanisms during P. marneffei infection. We found that ERK1/2 activation was dependent on the calcium/calmodulin/calmodulin kinase II pathway in P. marneffei-infected human macrophages. In contrast, P. marneffei-induced p38 MAPK activation was negatively regulated by calcium/calmodulin/calmodulin kinase II signaling pathway. Furthermore, TNF-alpha production in P. marneffei-infected human macrophages was also dependent on Ca2+/calmodulin/calmodulin kinase II pathway. These data suggest that Ca2+/calmodulin/calmodulin kinase II pathway plays vital regulatory roles in macrophage activation and subsequent cytokine production during P. marneffei infection.

• 出版日期2016-4
• 单位中山大学