Down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer cells by up-regulating EN2

作者：Zhou, Yao-jun; Yang, Hong-qiong; Xia, Wei; Cui, Li; Xu, Ren-fang; Lu, Hao; Xue, Zhong; Zhang, Bo; Tian, Zi-nong; Cao, Yun-jie; Xing, Zhao-yu; Yin, Shuai; He, Xiao-zhou^*

来源：Life Sciences, 2017, 190: 7-14.

DOI：10.1016/j.lfs.2017.09.028

• PCa
• EN2
• miR-605
• PCa cells

Aims: MicroRNA served as inhibitor for gene expression in various cancers. This study aimed to investigate the role of miR-605 and EN2 in prostate cancer (PCa). @@@ Materials and methods: In this research, the expression of miR-605 and EN2 protein in PCa tissues and cells were determined by qRT-PCR and western blot, respectively. The cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and the tumor cell invasion assay was accomplished with transwell system. Flow cytometry was used to analyze the cell cycle. The endogenous expression of miR-605 and EN2 was modulated by recombinant plasmids and cell transfection. Dual luciferase reporter assay was performed to determine the interaction between miR-605 and EN2 in PCa cells. @@@ Key findings: The expression of miR-605 was lower in PCa tissue and cells than that in normal tissues and cells, while the expression of EN2 was just the opposite. Down-regulation of the EN2 by siRNA inhibited the proliferation and invasion of PC3 cells, and the cell cycle was arrested in G0/G1 phase. EN2 regulated the expression of E-cadherin and Vimentin through Snail and EN2 regulated the cell cycle and cell proliferation via PI3K/AKT pathway. MiR-605 inhibited the proliferation and invasion of PCa cells through targeting EN2. @@@ Significance: EN2 is negatively regulated by miR-605, and down-regulation of miR-605 promotes the proliferation and invasion of PCa cells by up-regulating EN2, which leads to PCa development and progression.

• 出版日期2017-12-1
• 单位苏州大学