科研之友
微信
新浪微博
Facebook
分享链接
摘要
Background: Obesity is a major susceptibility factor leading to the development of various conditions of the metabolic syndrome. In obese rats, slowing of motor nerve conduction velocity was observed. Fatty acids metabolism disturbance is very important in the occurrence of peripheral neuropathy. The aim of this work is to consider the role that balanced diets high in omega 6%269 PUFA (corn oil) or supplying rats with omega 3, play in modulating the impaired nerve function in obese rats. Methods: Thirty two adult male albino rats were randomly assigned to receive normal chow (NC) (n=8) or high fat diet HFD (n=24), for 12 weeks. After 12 weeks, body weight and body mass index(BMI) were measured and the NC group(n=8) continue their normal chow diet, Group 1 (NC) and served as a control group and the obese rats were randomly divided into 3 groups, 8 rats each: Group 2: Ob + HFD group, they continue their high animal fat diet, Group 3: Ob+HFD + corn oil group, they are obese rats received high fat diet containing corn oil and Group 4: Ob + HFD + Omega 3 group, they are obese rats, fed high animal fat diet supplemented with omega 3 (0.4 g/kg) daily. After five weeks, the final body weight was measured and BMI was calculated and blood samples were collected for measuring fasting plasma glucose level, insulin level and homeostasis model assessment of insulin resistance (HOMA-IR) test were evaluated. Plasma cholesterol, triglycerides and free fatty acids (FFAs) were measured. The rats were then killed and sciatic nerves were carefully dissected for measuring the nerve conduction velocity (NCV). Superoxide dismutase activity (SOD), malondialdehyde (MDA) and tumor necrosis factor alpha (TNF alpha) were estimated in the nerve tissue of the 4 groups. Results: The results of this study showed a significant increase of body weight (gm) and BMI (kg/m(2)) in high fat diet group (p%26lt; 0.05) after 12weeks of the start of the diet when compared to the control group (NC). There were significant elevations in the final weight (gm) and BMI (kg/m(2)), a significant elevation in insulin level (mu IU/l) and HOMA-IR test, a significant increase in nerve malondialdehyde (MDA), and tumor necrosis factor alpha (TNF alpha) and a significant decrease in superoxide dismutase activity (SOD) and nerve conduction velocity (NCV) (m/s) after 5weeks of high fat diet in (Ob+HFD) group, when compared to NC group. Changing diet composition for 5weeks in Ob+ HFD+corn oil and Ob+HFD+omega 3 groups, did not induce any significant variation in body weight, BMI, or fasting blood glucose level as compared to Ob+HFD group. Insulin level (mu IU/l) and HOMA-IR test were significantly decreased in Ob+ HFD+corn oil and Ob+HFD+omega 3 groups compared to Ob+HFD group. Plasma cholesterol levels (mg/dl), triglycerides (mg/dl), and free fatty acids (FFA) (mmol/l) were significantly decreased after 5weeks diet in Ob+ HFD+corn oil or Ob+HFD+ Omega 3 groups when compared to mean values of Ob+HFD group. Tissue malondialdehyde (MDA) and tumor necrosis factor alpha (TNF alpha) were significantly decreased but superoxide dismutase (SOD) activity was significantly increased in Ob+HFD+corn oil and Ob+HFD+omega3 groups compared to Ob+HFD. NCV(m/s) in Ob+HFD+ corn oil group was significantly increased compared to Ob+ HFD and their values in Ob+HFD+ corn oil group showed no significant variation as compared to NC group. While there was a significant increase in NCV in Ob+ HFD+Omega 3 group as compared to Ob+ HFD group, there was still a significant decrease compared to NC group. %26lt;br%26gt;Conclusion: The results of this study may have important clinical and speculative implications. Corn oil or omega 3 supplementation may be effective in obesity induced neuropathy. The mechanism of their effects is multifactorial including improving insulin sensitivity, correction of dyslipdemia, reducing oxidative stress and an anti-inflammatory effect. This possibility should be carefully considered and examined in future trials of essential fatty acid supplementation. [Laila Ahmed El sayed, Samah Elattar, and Nashwa Eltablawy. Nerve Conduction Velocity of Sciatic Nerve in High Fat Diet Induced Obesity in Rats: Effect of Corn Oil and Omega 3 Fatty Acids Supplement. Life Sci J 2012;9(4):458-471] (ISSN:1097-8135). http://www.lifesciencesite.com. 68
- 出版日期2012
