A chimeric peptide that binds to titanium and mediates MC3T3-E1 cell adhesion

作者：Wang Dan; Mao Jing; Zhou Bin^*; Liao Xiao Fu; Gong Shi Qiang; Liu Yan; Zhang Jing Tao

来源：Biotechnology Letters, 2011, 33(1): 191-197.

DOI：10.1007/s10529-010-0411-9

• Cell adhesion
• Chimeric peptide
• MC3T3-E1 cell
• Titanium

Purpose of work Our study provides a promising alternative of biomimetic coating which functionalizes the dental implant with adhesion peptides and may be useful for enhancing the bone remodeling around Ti implants. A chimeric peptide consisting of an Arg-Gly-Asp (RGD) sequence (mediating cell adhesion) and a RKLPDA (minTBP-1) sequence (specifically recognizing and binding to Ti substrate) was designed and synthesized. The chimeric peptide affinity to Ti disks, as well as its role in mediating MC3T3-E1 cell attachment and afterwards spreading on pre-coated Ti disks, was investigated. The chimeric peptide not only showed favorable affinity to Ti surfaces but also facilitated the adhesion of MC3T3-E1 cells.

• 出版日期2011-1
• 单位华中科技大学