Analysis of methylation patterns and expression profiles of p14(ARF) gene in patients with oral squamous cell carcinoma

作者：Kordi Tamandani Dor Mohammad; Moazeni Roodi Abdolkarim; Ladez Mohammad Ayoub Rigi; Hashemi Mohammad; Birjandian Elnaz; Torkamanzehi Adam^*

来源：International Journal of Biological Markers, 2010, 25(2): 99-103.

• Oral squamous cell carcinoma
• p14(ARF) gene
• Promoter methylation
• Gene expression

Aims: To analyze the promoter methylation profile and mRNA expression of the p14(ARF) gene in oral squamous cell carcinoma (OSCC).
Methods: Promoter methylation of the p14(ARF) gene was investigated by methylation-specific polymerase chain reaction in paraffin-embedded tissues from 76 patients with OSCC and 57 oral tissues used as healthy controls. Expression of p14(ARF) mRNA was also determined using real-time quantitative reverse-transcription PCR. The methylation status and mRNA level profile of the gene and their relationship with clinical data were analyzed.
Results: Methylation of the p14(ARF) gene in OSCC was significantly increased compared to normal control tissues (chi(2) = 16.73, p < 0.0001). The relative expression of p14(ARF) mRNA in OSCC was not significantly different from that in healthy control samples.
Conclusion: Promoter methylation of p14(ARF) may be an important mechanism in OSCC, and its determination may be considered an important tool in the early diagnosis and treatment of OSCC.

• 出版日期2010-6