A new 4.0-generation dendrimer phosphorescence labeling reagent and its application to determination of trace alkaline phosphatase by affinity adsorption solid substrate-room temperature phosphorimetry

作者：Li Zhi Ming; Liu Jia Ming; Chen Xiao Hua; Yang Min Lan; Chen Xin Hua; Shi Xiu Mei

来源：Chinese Journal of Chemistry, 2007, 25(10): 1529-1535.

DOI：10.1002/cjoc.200790281

• alkaline phosphatase
• 4.0-generation dendrimer
• label
• wheat germ agglutinin
• affinity adsorption solid substrate-room temperature phosphorimetry

A Triton X-100-4.OG-D (4.OG-D refers to a 4.0-generation dendrimer) was brought forward as a new phosphorescence labeling reagent. Two types of specific affinity adsorption (AA) reactions (direct method and sandwich method) were carried out between the labeling product of Triton X-100-4.0G-D-Wheat germ agglutinin (WGA) and alkaline phosphatase (ALP), the product of AA reaction preserved the good characteristics of room temperature phosphorescence (RTP) of 4.OG-D and Delta/p of the product was proportional to the content of ALP. According to the fact stated above, a new method for the determination of trace ALP by affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) was established on the basis of WGA labeled with the Triton X- 100-4.0G-D. The detection limits were 0.20 ag center dot spot(-1) (corresponding concentration: 5.0 X 10(-16) g center dot mL(-1), namely 5.0 X 10(-8) mol center dot L-1) for a direct method and 0.14 ag center dot spot(-1) (corresponding concentration: 3.5 X 10(-16) g center dot mL(-1), namely 3.5 X 10(-18) mol center dot L-1) for a sandwich method, respectively. For their high sensitivity, good repeatability and high accuracy, the direct method and sandwich method have been successfully applied to determine the content of ALP in human serum, and the results were coincided with the clinical detection results of the enzyme-linked immunosorbent assay method by the Zhangzhou Hospital of Traditional Chinese Medicine. Meanwhile, the mechanism for the determination of trace ALP by AA-SS-RTP was discussed.

• 出版日期2007-10
• 单位漳州职业技术学院; 闽南师范大学