MicroRNA-29b Promotes the Adipogenic Differentiation of Human Adipose Tissue-Derived Stromal Cells

作者：Zhang, Xi-Mei^*; Wang, Li-Hong; Su, Dong-Ju; Zhu, Dan; Li, Qiu-Ming; Chi, Mei-Hua

来源：Obesity, 2016, 24(5): 1097-1105.

DOI：10.1002/oby.21467

• MESENCHYMAL STEM-CELLS
• ADIPOCYTE DIFFERENTIATION
• NEGATIVE REGULATOR
• PPAR-GAMMA
• EXPRESSION
• OBESITY
• MODULATION

Objective: Obesity is primarily characterized by the accumulation of large amounts of fat in adipose tissue. Within the adipose tissue, adipocytes are derived from adipose tissue-derived stromal cells (ADSCs) via a specialized cell lineage differentiation process, and ADSCs play a key role in the generation and metabolism of adipose tissue. This study investigated whether microRNAs (miRNAs) play a role in adipocyte differentiation. @@@ Methods: Using luciferase reporter and ChIP assays, the relationship between miR-29b, SP1, and TNF-alpha was examined. @@@ Results: During the normal adipogenic differentiation of ADSCs, up-regulation of miR-29b promoted adipogenesis by enhancing SP1-mediated inhibition of TNF-alpha. @@@ Conclusions: This study investigated the regulatory role of miR-29b during the adipogenic differentiation of ADSCs and found that miR-29b is an effective positive regulator of adipogenesis.

• 出版日期2016-5
• 单位哈尔滨医科大学