科研之友
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摘要
Background: Propionibacterium acnes (P. acnes) is a commensal bacterium which is possibly involved in acne inflammation. The saturated fatty acid, lauric acid (C12:0) has been shown to possess antibacterial and anti-inflammatory properties against P. acnes. Little is known concerning the potential effects of its decanoic counterpart, capric acid (C10:0). %26lt;br%26gt;Objective: To examine the antibacterial and anti-inflammatory activities of capric acid against P. acnes and to investigate the mechanism of the anti-inflammatory action. %26lt;br%26gt;Methods: The antimicrobial activity of fatty acids was detected using the broth dilution method. An evaluation of P. acnes-induced ear edema in mice was conducted to evaluate the in vivo anti-inflammatory effect. To elucidate the in vitro anti-inflammatory effect, human SZ95 sebocytes and monocytic THP-1 cells were treated with P. acnes alone or in the presence of a fatty acid. The mRNA levels and secretion of pro-inflammatory cytokines were measured by qRT-PCR and enzyme immunoassay, respectively. NF-kappa B activation and MAPK expression were analyzed by ELISA and Western blot, respectively. %26lt;br%26gt;Results: Lauric acid had stronger antimicrobial activity against P. acnes than capric acid in vitro and in vivo. However, both fatty acids attenuated P. acnes-induced ear swelling in mice along with microabscess and significantly reduced interleukin (IL)-6 and CXCL8 (also known as IL-8) production in P. acnesstimulated SZ95 sebocytes. P. acnes-induced mRNA levels and secretion of IL-8 and TNF-alpha. in THP-1 cells were suppressed by both fatty acids, which inhibited NF-kappa B activation and the phosphorylation of MAP kinases. %26lt;br%26gt;Conclusion: Our data demonstrate that both capric acid and lauric acid exert bactericidal and antiinflammatory activities against P. acnes. The anti-inflammatory effect may partially occur through the inhibition of NF-kappa B activation and the phosphorylation of MAP kinases.
- 出版日期2014-3
