Anatomical Identification of Extracellularly Recorded Cells in Large-Scale Multielectrode Recordings

作者：Li Peter H; Gauthier Jeffrey L; Schiff Max; Sher Alexander; Ahn Daniel; Field Greg D; Greschner Martin; Callaway Edward M; Litke Alan M; Chichilnisky E J^*

来源：Journal of Neuroscience, 2015, 35(11): 4663-4675.

DOI：10.1523/JNEUROSCI.3675-14.2015

• ganglion cells
• immunohistochemistry
• morphology
• multielectrode array
• retina
• viral transfection

This study combines for the first time two major approaches to understanding the function and structure of neural circuits: large-scale multielectrode recordings, and confocal imaging of labeled neurons. To achieve this end, we develop a novel approach to the central problem of anatomically identifying recorded cells, based on the electrical image: the spatiotemporal pattern of voltage deflections induced by spikes on a large-scale, high-density multielectrode array. Recordings were performed from identified ganglion cell types in the macaque retina. Anatomical images of cells in the same preparation were obtained using virally transfected fluorescent labeling or by immunolabeling after fixation. The electrical image was then used to locate recorded cell somas, axon initial segments, and axon trajectories, and these signatures were used to identify recorded cells. Comparison of anatomical and physiological measurements permitted visualization and physiological characterization of numerically dominant ganglion cell types with high efficiency in a single preparation.

• 出版日期2015-3-18