A novel single-stranded RNA virus isolated from the rice-pathogenic fungus Magnaporthe oryzae with similarity to members of the family Tombusviridae

作者：Ai, Ye-Ping; Zhong, Jie; Chen, Chuan-Yuan; Zhu, Hong-Jian^*; Gao, Bi-Da^*

来源：Archives of Virology, 2016, 161(3): 725-729.

DOI：10.1007/s00705-015-2683-9

• BLAST FUNGUS
• NUCLEOTIDE-SEQUENCE
• VEGETATIVE GROWTH
• STOP CODON
• PLANT
• MYCOVIRUSES
• READTHROUGH
• EXPRESSION
• PROTEINS

Here, we report a novel virus isolated from rice blast fungus, Magnaporthe oryzae, an important plant pathogen. This virus has an RNA genome of 3246 nucleotides. Its genome possesses two in-frame open reading frames (ORFs). The smaller ORF1 encodes a protein with significant similarity to a protein encoded by the ssRNA mycovirus Diaporthe ambigua RNA virus 1 (DaRV1). The larger ORF2 encodes a protein with similarity to RNA-dependent RNA polymerases (RdRp) of DaRV1 and other plant viruses of the family Tombusviridae. In silico analysis and comparisons with DaRV1 genome expression suggest that ORF2 is translated via a readthrough mechanism together with ORF1. Based upon results of this study, this virus, for which the provisional name Magnaporthe oryzae virus A (MoVA) is proposed, belongs to a new virus species. Furthermore, MoVA along with DaRV1 belong to a new taxon of mycoviruses that are evolutionarily related to plant viruses belonging to the family Tombusviridae.

• 出版日期2016-3
• 单位湖南农业大学