Depressed production of beta-defensins from mouse splenic dendritic cells following thermal injury and its influence on susceptibility to infection

作者：Kawasaki Takashi^*; Kawasaki Chika; Sata Takeyoshi; Kobayashi Makiko; Suzuki Fujio

来源：Journal of Anesthesia, 2015, 29(1): 78-86.

DOI：10.1007/s00540-014-1882-y

• Burn
• Defensin
• Dendritic cell
• Infection
• Innate immunity
• Host defense

Purpose Beta-defensins (BDs) and dendritic cells (DC) have been described as major effectors on host antimicrobial innate immunities. In the present study, the ability of DC to produce BDs was explored using DC from normal mice and full-thickness (FT)-burned mice.
Methods DCs were isolated from spleens of mice, and 1 x 10(6) cells/ml of them were cultured with LPS or SAC. Culture fluids harvested 24 h after cultivation were assayed for BD1 and BD3 and antibacterial activity (colony-counting, Pseudomonas aeruginosa). Also, DCs were tested for BD mRNAs by RT-PCR.
Results Sixty-five percent of the bacterial killing activity was shown by the culture fluids of splenic DC from normal mice, while only 15 % killing activity was shown by the culture fluids of splenic DC from FT-burned mice. X-irradiated NOD SCID IL-2r gamma(null) mice inoculated with splenic DC from FT-burned mice showed increased susceptibility to P. aeruginosa infection compared to those from normal mice. Mice splenic DC expressed BD1 mRNA constitutively and expressed BD3 mRNA after stimulation. These BDs were produced by mice splenic DC. As compared with DC from normal mice, DC from FT-burned mice produced decreased amounts of BD1 and BD3 in their culture fluids.
Conclusions These results indicate that (1) DC from spleens of mice have an ability to produce BDs, and (2) the production of BDs by DC is influenced strongly by thermally injured stress. Since FT-burned mice are susceptible to P. aeruginosa infection, BDs produced by DC may play an important role on the host's antibacterial resistance.

• 出版日期2015-2