Application of BRET to monitor ligand binding to GPCRs

作者：Stoddart Leigh A; Johnstone Elizabeth K M; Wheal Amanda J; Goulding Joelle; Robers Matthew B; Machleidt Thomas; Wood Keith V; Hill Stephen J; Pfleger Kevin D G^*

来源：Nature Methods, 2015, 12(7): 661-+.

DOI：10.1038/NMETH.3398

Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

• 出版日期2015-7
• 单位河北医科大学