Direct miRNA-hybridization assays and their potential in diagnostics

作者：Wegman David W; Krylov Sergey N^*

来源：TrAC-Trends in Analytical Chemistry, 2013, 44: 121-130.

DOI：10.1016/j.trac.2012.10.014

• Diagnostics
• Direct assay
• Hybridization assay
• MicroRNA (miRNA)
• MicroRNA detection
• miRNA fingerprint
• Probe-miRNA duplex
• Sequencing-based method
• Signature-based method
• Spatial separation

MicroRNAs (miRNAs) play a significant role in gene regulation and have been shown to be deregulated in various diseases. Specific sets of deregulated miRNAs, termed %26quot;miRNA fingerprints%26quot;, can distinguish diseased from healthy samples. Detecting disease-specific fingerprints could be used in diagnostics and there are significant efforts towards developing miRNA-detection methods for this purpose. These methods would require the ability to detect multiple miRNAs in a direct, quantitative, specific, and rugged manner. %26lt;br%26gt;This review discusses potential diagnostic methods for miRNA detection, with the main focus on direct hybridization assays. MiRNA-hybridization assays are categorized into two groups, depending on whether or not they require spatial separation of the labeled probe-miRNA duplex from unbound probe. We find that spatial-separation hybridization assays are able to meet all of our criteria, so they have the most potential for use in diagnostics.

• 出版日期2013-3