A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections

作者：Lu Chun Yi; Chang Luan Yin^*; Chen Pei Jer; Xia Ning Shao; Shao Pei Lan; Huang Li Min

来源：Journal of the Formosan Medical Association, 2012, 111(12): 693-697.

DOI：10.1016/j.jfma.2011.11.029

• diagnosis
• ELISA
• pandemic
• 2009 Influenza A (H1N1)

Background/Purpose: Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples.
Methods: A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested.
Results: Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively.
Conclusion: The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity.

• 出版日期2012-12
• 单位厦门大学