Development of a Simple Sample Pretreatment Method for Capillary Electrophoretic Determination of Melamine in Milks

作者：Katou Yuuyichi^*; Wu Xing Zheng

来源：Bunseki Kagaku, 2012, 61(5): 419-423.

• capillary electrophoresis
• milk
• melamine
• pretreatment
• acetic acid

The determination of melamine in milk samples by capillary electrophoresis (CE) usually requires a complex sample pretreatment. Here, we show a simple sample pretreatment method for the capillary electrophoretic determination of melamine in milk samples with 0.125 mol/L acetic acid solution. It was found that melamine on the level of ppm in milk can be simply and rapidly determined by CE after a pretreatment with a 0.125 mol/L acetic acid solution. The detection limit of melamine was 1 ppm by the proposed method; also, the melamine concentration was proportional to its peak area. The relatively standard deviations of 10 determinations of a milk sample spiked with 10 ppm melamine were 1.0 % and 10.5 % for the electromigration time (peak time) and the peak area, respectively.

• 出版日期2012-5