ADP-Mg2+ bound to the ATP-grasp domain of ATP-citrate lyase

作者：Sun Tianjun; Hayakawa Koto; Fraser Marie E^*

来源：Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2011, 67: 1168-1172.

DOI：10.1107/S1744309111028363

Human ATP-citrate lyase (EC 2.3.3.8) is the cytoplasmic enzyme that catalyzes the production of acetyl-CoA from citrate, CoA and ATP. The amino-terminal portion of the enzyme, containing residues 1-817, was crystallized in the presence of tartrate, ATP and magnesium ions. The crystals diffracted to 2.3 angstrom resolution. The structure shows ADP-Mg2+ bound to the domain that possesses the ATP-grasp fold. The structure demonstrates that this crystal form could be used to investigate the structures of complexes with inhibitors of ATP-citrate lyase that bind at either the citrate-or ATP-binding site.

• 出版日期2011-10