科研之友
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摘要
The determination of uric acid has been carried out at pH 7.2 (mu = 0.1 M) at nanogold modified indium tin oxide electrode by differential pulse voltammnetry in absence and presence of ascorbic acid and other common interferents. A well - defined oxidation peak has been observed at around 840 mV vs Ag/AgCl electrode for uric acid at pH 7.2. The plot of peak current versus concentration plot is linear in the range 0.5 - 100 mu M with a correlation coefficient of 0.995 and a sensitivity of 0.0004 nA/mM. The detection limit (3 sigma) is found to be 0.5 mu M. Ascorbic acid in concentrations lower than 10 mu M does not interfere, whereas, at higher concentrations it interferes. Xanthine, hypoxanthine and adenine do not interfere even at eight-fold excess. The method is simple and convenient with the only limitation that the electrode cannot be used more than eight times.
- 出版日期2005-5
