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摘要
Ethnopharmacological relevance: Senna racemosa (Mill.) H.S. Irwin & Barneby (syn. Cassia racemosa Mill.) is a plant used in traditional Mayamedicinal practices to treat diarrhea. A methanol extract of S. racemosa bark has been shown to have in vitro activity against Giardia intestinalis. No studies of its efficacy and toxicity in in vivo models have been done. The present study objective was to analyze the activity of this methanol extract of S. racemosa bark against Giardia intestinalis trophozoites in experimentally infected mice, and evaluate its toxicological effects in rats. Material and methods: S. racemosa was collected in Merida, Yucatan, Mexico (21 degrees 58'N, 89 degrees 36'W) in June 2005. The bark methanol extract was obtained and high performance liquid chromatography (HPLC-DAD) was used to generate a constituent profile. In vivo anti-giardia activity was assayed with an experimental model of G. intestinalis infection in neonatal CD-1 mice. Nine doses ranging from 0.25-15 mg extract/kg body weight were tested to determine the dose required to kill 50% of the trophozoites (ED50). An acute toxicity assay was run in which one of four single doses (200, 1000, 2000 and3000 mg/kg body weight) was orally administered to adult Wistar rats. Animal weight, death rates, toxic effects and behavioral parameters were observed over a 14-d period. They were then euthanized and a necropsy performed. Results: The S. racemosa bark extract inhibited growth of G. intestinalis (ED50=1.14 mg/Kg) in neonatal CD-1 mice. No toxic or lethal effects were observed even at the highest dosage (3000 mg/Kg), and neither were signs of toxicity observed in internal organs. The active compounds chrysophanol and physcion were present in the extract at a 1.76 ratio. Conclusions: The results strongly support traditional use of S. racemosa bark for treatment of diarrhea caused by Giardia intestinalis infection.
- 出版日期2016-12-4
