An Arabidopsis Clathrin Assembly Protein with a Predicted Role in Plant Defense Can Function as an Adenylate Cyclase

作者：Chatukuta, Patience; Dikobe, Tshegofatso B.; Kawadza, David T.; Sehlabane, Katlego S.; Takundwa, Mutsa M.; Wong, Aloysius; Gehring, Chris; Ruzvidzo, Oziniel^*

来源：Biomolecules, 2018, 8(2): 15.

DOI：10.3390/biom8020015

• Arabidopsis thaliana
• adenylate cyclase
• cAMP
• clathrin assembly
• endocytosis
• pathogen responses

Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cyaA in E. coli, and a recombinant AtClAP fragment (AtClAP(261-379)) can produce cyclic adenosine 3,5 monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.

• 出版日期2018-6
• 单位Perugia; 西北大学