Novel superparamagnetic PFu@Fe3O4 conductive nanocomposite as a suitable host for hemoglobin immobilization

作者：Baghayeri Mehdi^*; Zare Ehsan Nazarzadeh; Lakouraj Moslem Mansour

来源：Sensors and Actuators B: Chemical , 2014, 202: 1200-1208.

DOI：10.1016/j.snb.2014.06.019

• Nanocomposite
• Biosensor
• Hemoglobin
• Hydrogen peroxide

A highly-sensitive hydrogen peroxide biosensor was fabricated by immobilization of hemoglobin (Hb) onto a biocompatible PFu@Fe3O4 nanocomposite/glassy carbon electrode (PFu@Fe3O4/GCE). The PFu@Fe3O4 nanocomposite was characterized by Fourier transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and vibrating sample magnetometer (VSM). PFu@Fe3O4 nanocomposite which was synthesized via in situ polymerization at room temperature combined the advantages of superparamagnetic properties from Fe3O4 nanoparticles and conductive poly(furan) (PF) as the building block for fabrication of biosensor. The Hb immobilized on PFu@Fe3O4 nanocomposite retained its near-native conformations as characterized by the FT-IR. The heterogeneous electron transfer rate constant and Michaelis-Menten constant were calculated to be 0.76 s(-1) and 0.094 mM at pH 7.0, respectively. The proposed biosensor exhibited a linear response to hydrogen peroxide in the concentration ranging from 0.5 mu M to 350.0 mM with a correlation coefficient of 0.995, sensitivity of -0.089 mu A mu M-1 and detection limit of 0.31 mu M (S/N = 3), which could make it a promising third-generation biosensor for precise detection of hydrogen peroxide in the biological samples.

• 出版日期2014-10