Anti-inflammatory effects of farrerol on IL-1 beta-stimulated human osteoarthritis chondrocytes

作者：Zhang Hongfei^*; Yan Jiapeng; Zhuang Yuesheng; Han Guiquan

来源：European Journal of Pharmacology, 2015, 764: 443-447.

DOI：10.1016/j.ejphar.2015.07.012

• Farrerol
• Osteoarthrais chondrocyte
• IL-1 beta
• NF-kappa B
• PI3K

The present study aimed to investigate the anti-inflammatory effects and the underlying molecular mechanism of farrerol on IL-1 beta-stimulated human osteoarthritis chondrocytes. Chondrocytes were pretreated with farrerol 1 h before IL-1 beta stimulation. The effects of farrerol on NO and PGE2 production were tested by Griess reagent and ELISA. The effects of farrerol on COX-2, iNOS, Akt, phosphorylated Akt, and NE-kappa B activation were measured by western blot analysis. The results showed that farrerol remarkably inhibited IL-1 beta-induced NO and PGE2 production, as well as COX-2 and iNOS expression. Farrerol also inhibited IL-1 beta-induced NF-kappa B activation. Furthermore, farrerol significantly inhibited IL-1 beta-induced phosphorylation of PI3K and Akt. In conclusion, these results indicated that farrerol inhibited IL-1 beta-induced inflammatory responses in osteoarthritis chondrocytes by blocking PI3K/Akt/NF-kappa B signaling pathway.

• 出版日期2015-10-5
• 单位潍坊医学院