A structural characterization of human SCO2

作者：Banci Lucia; Bertini Ivano^*; Ciofi Baffoni Simone; Gerothanassis Loannis P; Leontari Iliana; Martinelli Manuele; Wang Shenlin

来源：Structure, 2007, 15(9): 1132-1140.

DOI：10.1016/j.str.2007.07.011

Human Sco2 is a mitochondrial membrane-bound protein involved in copper supply for the assembly of cytochrome c oxidase in eukaryotes. Its precise action is not yet understood. We report here a structural and dynamic characterization by NMR of the apo and copper(I) forms of the soluble fragment. The structural and metal binding features of human Cu(I)Sco2 are similar to the more often studied Scot homolog, although the dynamic properties and the conformational disorder are quite different when the apo forms and the copper(I)-loaded forms of the two proteins are compared separately. Such differences are accounted for in terms of the different physicochemical properties in strategic protein locations. The misfunction of the known pathogenic mutations is discussed on the basis of the obtained structure.

• 出版日期2007-9