Grandisin induces apoptosis in leukemic K562 cells

作者：Cortez Alane Pereira; Paulino Menezes Elizabeth Gomes; Benfica Polyana Lopes; dos Santos Alexandre Pereira; Cleres Larissa Moreira; Ribeiro Higor de Oliveira; Lima Eliana Martins; Kato Massuo Jorge; Valadares Marize Campos^*

来源：Brazilian Journal of Pharmaceutical Sciences, 2017, 53(1): UNSP e15210.

DOI：10.1590/s2175-97902017000115210

• Grandisin/antileukemic activities
• Leukemic
• K562
• Apoptosis

In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 mu M) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85 mu M. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was confirmed by anexin V stain and caspase activation. Thus, lignan grandisin showed antileukemic activities against the K562 cell line and the cell death process occurred via apoptosis.

• 出版日期2017