An important role of interleukin-10 in counteracting excessive immune response in HT-29 cells exposed to Clostridium butyricum

作者：Gao Quanxin; Qi Lili; Wu Tianxing; Wang Jinbo^*

来源：BMC Microbiology, 2012, 12: 100.

DOI：10.1186/1471-2180-12-100

Background: Clostridium butyricum has become increasingly important in preventing and treating intestinal inflammation. In the intestine it may increase the resistance of the gut to pathogen invasion via inducing the secretion of anti-inflammatory cytokines. Interleukin 10 (IL-10) plays a central role in preventing certain inflammatory diseases by down-regulating inflammatory cascades. In a previous study, we observed that the level of IL-10 mRNA was modulated by C. butyricum. The aim of this study was to investigate whether C. butyricum achieves its beneficial effects through IL-10.
Results: We treated HT-29 cells with anti-IL-10 (IL-10 antibody) or siIL-10 (IL-10 small interfering RNA) to disrupt IL-10. In both cases, the effects of C. butyricum-induced NF-kappa B activation and IL-8 expression were enhanced. We also found that neutralization or knockdown of IL-10 could induce apoptosis and necrosis of HT-29 cells treated with C. butyricum compared with control cells.
Conclusions: These findings show that IL-10 serves an important role in C. butyricum-mediated immune protection, and in host recognition of C. butyricum.

• 出版日期2012-6-8
• 单位浙江大学宁波理工学院; 浙江大学