A whisker-like carbon composite for the immobilization of laccase and its bioelectrochemistry

作者：Ma GuoXian^*; Zhong QingDong; Lu TianHong

来源：Science China Chemistry, 2010, 53(6): 1332-1336.

DOI：10.1007/s11426-010-3186-x

• laccase
• whisker-like carbon composite
• direct electrochemical reaction
• oxygen
• bioelectrochemistry

A novel mesoporous carbon/whisker-like carbon (MCWC) composite was used for the immobilization of laccase ( Lac) and its bioelectrochemical behaviors were studied. It was confirmed by XPS that Lac was strongly adsorbed on the surface of the MCWC composite. The cyclic voltammetric results showed that the immobilized Lac underwent a direct quasi-reversible electrochemical reaction. The value of the electron transfer rate constant k(s) was estimated to be 0.770 s(-1), indicating a reasonably fast electron transfer between the immobilized Lac and the underlying electrode. The surface concentration (Gamma) of Lac was estimated to be 2.730 x 10(-12) mol/cm(2). Further experimental results showed that the immobilized Lac displayed an appreciable electrocatalytic activity to the electrochemical reduction of O(2). These properties could be attributed to the particular structure of loosely packed nanometer-scale carbon whiskers and the existence of a large amount of oxygen-containing groups. The immobilization method and the novel carrier (MCWC) may find new applications in fabricating the biocatalysts for biofuel cells.

• 出版日期2010-6
• 单位上海应用技术大学; 南京师范大学; 上海大学