Melatonin, melatonin isomers and stilbenes in Italian traditional grape products and their antiradical capacity

作者：Vitalini Sara; Gardana Claudio; Simonetti Paolo; Fico Gelsomina; Iriti Marcello^*

来源：Journal of Pineal Research, 2013, 54(3): 322-333.

DOI：10.1111/jpi.12028

• antioxidant activity
• grape juice
• melatonin isomers
• Modena balsamic vinegar
• polyphenols
• stilbenes
• traditional Mediterranean diet
• wine

Although polyphenols represent the paradigm of the health-promoting effects ascribed to grape products, recently, attention has been paid to dietary melatonin, significantly present in Mediterranean foods. In this work, we measured melatonin, its isomers, stilbenes (trans- and cis-resveratrol and their glucosides, piceids) and total polyphenols in some different grape products (red, white and dessert wines, grape juices and Modena balsamic vinegars) of distinct Italian areas. We also evaluated their antiradical activity by DPPH center dot and ABTS center dot+ assays. For indoleamine analysis, the separation was carried out on a 1.7-m C18 BEH column and the detection performed by means of mass spectrometry with electrospray ionization in positive ion mode with multiple reaction monitoring. The confirmation of the peak identity was accomplished by injection into the high-resolution system (Orbitrap) using accurate mass measurements (error below 1.0ppm). Mass spectrometry analyses revealed, for the first time, the presence of melatonin in dessert wines and balsamic vinegars, as well as the occurrence of three different melatonin isomers in grape products.

• 出版日期2013-4