A Microfluidic Chamber To Study the Dynamics of Muscle-Contraction-Specific Molecular Interactions

作者：Roman Horia Nicolae; Juncker David; Lauzon Anne Marie^*

来源：Analytical Chemistry, 2015, 87(5): 2582-2587.

DOI：10.1021/ac503963r

In vitro motility and laser trap assays are commonly used for molecular mechanics measurements. However, chemicals cannot be added during these measurements, because they create flows that alter the molecular mechanics. Thus, we designed a microfluidic device that allows the addition of chemicals without creating bulk flows. Biocompatibility of the components of this device was tested. A microchannel chamber was created by photolithography with the patterns transferred to polydimethylsiloxane (PDMS). The PDMS chamber was bound to a polycarbonate membrane, which itself was bound to a molecular mechanics chamber. The microchannels ensured rapid distribution of the chemicals over the membrane, whereas the membrane ensured efficient delivery to the mechanics chamber while preventing bulk flow. The biocompatibility of the materials was tested by comparing the velocity (nu(max)) of propulsion by myosin of fluorescently labeled actin filaments to that of the conventional assay; no difference in nu(max) was observed. To estimate total chemical delivery time, labeled bovine serum albumin was injected in the channel chamber and TIRF was used to determine the time to reach the assay surface (2.7 +/- 0.1 s). Furthermore, the standard distance of a trapped microsphere calculated during buffer diffusion using the microfluidic device (14.9 +/- 3.2 nm) was not different from that calculated using the conventional assay (15.6 +/- 5.3 nm, p = 0.922). Finally, nu(max) obtained by injecting adenosine triphosphate (ATP) in the microchannel chamber (2.37 +/- 0.48 mu m/s) was not different from that obtained when ATP was delivered directly to the mechanics chamber (2.52 +/- 0.42 mu m/s, p = 0.822). This microfluidic prototype validates the design for molecular mechanics measurements.

• 出版日期2015-3-3