One-step fabrication of sub-10-nm plasmonic nanogaps for reliable SERS sensing of microorganisms

作者：Chen Jing; Qin Gaowu^*; Wang Jiansheng; Yu Jiangyu; Shen Bo; Li Song; Ren Yuping; Zuo Liang; Shen Wen; Das Biswajit

来源：Biosensors and Bioelectronics, 2013, 44: 191-197.

DOI：10.1016/j.bios.2013.01.038

• Biosensor
• High pressure sputtering
• Microorganisms
• Sub-10-nm nanogaps
• Surface enhanced Raman scattering

Nanoscale gaps in noble metal films can produce intense electromagnetic enhancement. When Raman-active molecules are positioned in these regions, their surface-enhanced Raman scattering (SERS) signals can be dramatically enhanced. However, the lack of convenient and reliable fabrication methods with ultrasmall nanogaps (< 10 nm) severely block the application of SERS. Here, we propose a cost-effective and reproducible technique to fabricate the large-area Ag SERS-active substrates which are full of the high-density, sub-10-nm nanogaps by high pressure sputtering, and the enhancement factor (EF) is testified to improve by 10(3) times compared to the continuous Ag film with a smooth surface (the roughness is 0.5 nm) and without nanogaps. Since there are no chemicals used during fabrication, this substrate has a clean surface, which is crucial for acquiring reliable SERS spectra. This SERS-active substrate has then been applied to identify a series of microorganisms, and excellent, reproducible SERS spectra were obtained. Finally, a set of piecewise-linear equations is provided according to the correlation between SERS intensity and rhodamine 6G (R6G) concentration, and the detection limit is calculated to be 0.2 x 10(-8) M. These results suggest that the high pressure sputtering is an excellent, reliable technique for fabricating sub-10-nm plasmonic nanogaps, and the SERS-based methodology is very promising for being used in biological sensing field.

• 出版日期2013-6-15
• 单位东北大学