Analytical determination and pharmacokinetics of major metabolites of carbasalate calcium in broilers following oral administration

作者：Wang, X.; Huang, L. -L.; Chen, D. -M.; Ihsan, A.; Yuan, Z. -H.^*

来源：Journal of Veterinary Pharmacology and Therapeutics, 2011, 34(4): 410-416.

DOI：10.1111/j.1365-2885.2010.01250.x

• PERFORMANCE LIQUID-CHROMATOGRAPHY
• DIETARY ACETYLSALICYLIC-ACID
• TANDEM MASS-SPECTROMETRY
• SALICYLIC-ACID
• GENTISIC ACID
• HUMAN-PLASMA
• SODIUM-SALICYLATE
• SENSITIVE DETERMINATION
• EGGSHELL QUALITY
• HUMAN URINE

As a newer anti-inflammatory agent, carbasalate calcium is used in various animal species. In this study, the pharmacokinetics of carbasalate calcium was investigated in broilers. Broilers, with body weight of 2.0 +/- 0.3 kg, were administrated carbasalate calcium soluble powder at a single dose of 40 mg/kg body weight orally. The plasma concentrations of its metabolites, aspirin (ASA), salicylic acid (SA) and gentisic acid (GA) were determined by LC-MS/MS method and the pharmacokinetic parameters were calculated by noncompartmental analysis. After oral administration of carbasalate calcium, the plasma drug concentration for ASA, SA and GA reached a peak (C-max) of 8.88 +/- 1.31, 42.6 +/- 4.62 and 10.1 +/- 2.16 mu g/mL at 0.170, 2.00 and 2.00 h, respectively. The terminal half-life (t(1/2 lambda z)) of ASA, SA and GA was 11.2 +/- 8.04, 23.7 +/- 17.1 and 28.6 +/- 4.90 h, respectively. In conclusion, analytical method for the quantification of ASA, SA and GA in plasma in the broilers was developed and validated. In broilers, carbasalate calcium is quickly metabolized in ASA and ASA is rapidly converted to SA and one of the metabolites of SA is GA.

• 出版日期2011-8
• 单位华中农业大学