A critical role for endoglin in the emergence of blood during embryonic development

作者：Borges Luciene; Iacovino Michelina; Mayerhofer Timothy; Koyano Nakagawa Naoko; Baik June; Garry Daniel J; Kyba Michael; Letarte Michelle; Perlingeiro Rita C R^*

来源：Blood, 2012, 119(23): 5417-5428.

DOI：10.1182/blood-2011-11-391896

Much remains unknown about the signals that induce early mesoderm to initiate hematopoietic differentiation. Here, we show that endoglin (Eng), a receptor for the TGF beta superfamily, identifies all cells with hematopoietic fate in the early embryo. These arise in an Eng(+)Flk1(+) mesodermal precursor population at embryonic day 7.5 (E7.5), a cell fraction also endowed with endothelial potential. In Eng-knockout embryos, hematopoietic colony activity and numbers of CD71(+) Ter119(+) erythroid progenitors were severely reduced. This coincided with severely reduced expression of embryonic globin and key bone morphogenic protein (BMP) target genes, including the hematopoietic regulators Scl, Gata1, Gata2, and Msx-1. To interrogate molecular pathways active in the earliest hematopoietic progenitors, we applied transcriptional profiling to sorted cells from E7.5 embryos. Eng(+)Flk-1(+) progenitors coexpressed TGF beta and BMP receptors and target genes. Furthermore, Eng(+)Flk-1(+) cells presented high levels of phospho-SMAD1/5, indicating active TGF beta and/or BMP signaling. Remarkably, under hematopoietic serum-free culture conditions, hematopoietic outgrowth of Eng-expressing cells was dependent on the TGF beta superfamily ligands BMP4, BMP2, or TGF-beta 1. These data demonstrate that the E+F+ fraction at E7.5 represents mesodermal cells competent to respond to TGF beta 1, BMP4, or BMP2, shaping their hematopoietic development, and that Eng acts as a critical regulator in this process by modulating TGF/BMP signaling. (Blood. 2012;119(23):5417-5428)

• 出版日期2012-6-7