A metabolic checkpoint protein GlmR is important for diverting carbon into peptidoglycan biosynthesis in Bacillus subtilis

作者：Patel, Vaidehi; Wu, Qun; Chandrangsu, Pete; Helmann, John D.^*

来源：PLoS Genetics, 2018, 14(9): e1007689.

DOI：10.1371/journal.pgen.1007689

• C-DI-AMP
• ESCHERICHIA-COLI
• CATABOLITE REPRESSION
• N-ACETYLGLUCOSAMINE
• CELL-ENVELOPE
• SMALL RNA
• N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE
• KINETIC CHARACTERIZATION
• FEEDBACK INHIBITION
• GROWTH-CONDITIONS

The Bacillus subtilis GlmR (formerly YvcK) protein is essential for growth on gluconeogenic carbon sources. Mutants lacking GlmR display a variety of phenotypes suggestive of impaired cell wall synthesis including antibiotic sensitivity, aberrant cell morphology and lysis. To define the role of GlmR, we selected suppressor mutations that ameliorate the sensitivity of a glmR null mutant to the beta-lactam antibiotic cefuroxime or restore growth on gluconeogenic carbon sources. Several of the resulting suppressors increase the expression of the GlmS and GlmM proteins that catalyze the first two committed steps in the diversion of carbon from central carbon metabolism into peptidoglycan biosynthesis. Chemical complementation studies indicate that the absence of GImR can be overcome by provision of cells with N-acetylglucosamine (GlcNAc), even under conditions where GlcNAc cannot re-enter central metabolism and serve as a carbon source for growth. Our results indicate that GlmR facilitates the diversion of carbon from the central metabolite fructose-6-phosphate, which is limiting in cells growing on gluconeogenic carbon sources, into peptidoglycan biosynthesis. Our data suggest that GlmR stimulates GlmS activity, and we propose that this activation is antagonized by the known GlmR ligand and peptidoglycan intermediate UDP-GlcNAc. Thus, GlmR presides over a new mechanism for the regulation of carbon partitioning between central metabolism and peptidoglycan biosynthesis.

• 出版日期2018-9
• 单位江南大学