Highly efficient gene expression of a fibrinolytic enzyme (subtilisin DFE) in Bacillus subtilis mediated by the promoter of alpha-amylase gene from Bacillus amyloliquefaciens

作者：Xiao L; Zhang RH; Peng Y; Zhang YZ^*

来源：Biotechnology Letters, 2004, 26(17): 1365-1369.

DOI：10.1023/B:BILE.0000045634.46909.2b

• alpha-amylase gene promoter
• Bacillus amyloliquefaciens
• Bacillus subtilis
• douchi fibrinolytic enzyme gene
• gene expression

Subtilisin DFE is a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4. The promoter and signal peptide-coding sequence of alpha-amylase gene from B. amyloliquefaciens was cloned and fused to the sequence coding for pro-peptide and mature peptide of subtilisin DFE. This hybrid gene was inserted into the Escherichia coli/Bacillus subtilis shuttle plasmid vector, pSUGV4. Recombinant subtilisin DFE gene was successfully expressed in B. subtilis WB600 with a fibrinolytic activity of 200 urokinase units ml(-1).

• 出版日期2004-9
• 单位四川大学