A MAS NMR Study of the Bacterial ABC Transporter ArtMP

作者：Lange Vivien; Becker Baldus Johanna; Kunert Britta; van Rossum Barth Jan; Casagrande Fabio; Engel Andreas; Roske Yvette; Scheffel Frank M; Schneider Erwin; Oschkinat Hartmut^*

来源：ChemBioChem, 2010, 11(4): 547-555.

DOI：10.1002/cbic.200900472

• 2D and 3D crystals
• ABC transporter
• membrane proteins
• selective isotope labeling
• solid-state magic-angle spinning NMR spectroscopy

ATP-binding cassette (ABC) transport systems facilitate the translocation of substances, like amino acids, across cell membranes energised by ATP hydrolysis. This work describes first structural studies on the ABC transporter ArtMP from Geobacillus stearothermophilus in native lipid environment by magic-angle spinning NMR spectroscopy. The 2D crystals of ArtMP and 3D crystals of isolated ArtP were prepared in different nucleotide-bound or -unbound states. From selectively (13)C,(15)N-labelled ArtP, several sequence-specific assignments were obtained, most of which could be transferred to spectra of ArtMP. Residues Tyr133 and Pro134 protrude directly into the ATP-binding pocket at the interface of the ArtP subunits, and hence, are sensitive monitors for structural changes during nucleotide binding and hydrolysis. Distinct sets of NMR shifts were obtained for ArtP with different phosphorylation states of the ligand. Indications were found for an asymmetric or inhomogeneous state of the ArtP dimer bound with triphosphorylated nucleotides. With this investigation, a model system was established for screening all functional states occurring in one ABC transporter in native lipid environment.

• 出版日期2010-3-1