Analysis of QTL for yield-related traits in cassava using an F-1 population from non-inbred parents

作者:Chen, Xin; Fu, Yuhua; Xia, Zhiqiang; Jie, Li; Wang, Haiyan; Lu, Cheng; Wang, Wenquan*
来源:Euphytica, 2012, 187(2): 227-234.
DOI:10.1007/s10681-012-0662-8

摘要

A cassava F-1 population raised from the cross SC6 x Mianbao was used to construct a genetic linkage map. The map incorporated 200 polymorphic amplified fragment length polymorphism, sequence-related amplified polymorphism, simple sequence repeat (SSR), and expressed sequence tag (EST)-SSR markers which fit a 1:1 segregation ratio. It comprised 20 linkage groups (LGs) and spanned a genetic distance of 1645.1 cM with an average marker interval of 8.2 cM. Fifty-seven repeatedly detected QTLs (rd-QTLs) for three phenotypic traits (fresh root yield, root dry matter content, and root starch content) were identified in the F-1 population in four trials of year 2003, 2004, 2005, and 2008 by inclusive composite interval mapping. Among the 57 rd-QTLs, 25 rd-QTLs were linked to SSR/EST-SSR markers, which will help to facilitate marker-assisted selective breeding in cassava, and 15 marker intervals on ten LGs showed pleiotropic effects.

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