DETECTION OF HERPES-SIMPLEX VIRUS TYPE-2 BGL-II N-FRAGMENT IN PARAFFIN-EMBEDDED CERVICAL TISSUE-SECTIONS USING NESTED POLYMERASE CHAIN-REACTION

作者：LULITANOND V; CHANTRATITA W; THAMMAPRASERT K; NIMMANAHAEMINDA K; MATANGKASOMBUT P; YOOSOOK C

来源：Molecular and Cellular Probes, 1994, 8(6): 441-447.

DOI：10.1006/mcpr.1994.1063

• HERPES SIMPLEX VIRUS TYPE 2 BGL II N FRAGMENT
• CERVICAL CARCINOMA
• NESTED POLYMERASE CHAIN REACTION
• OLIGONUCLEOTIDE PROBE
• 3'-END LABELING
• CARRYOVER CONTAMINATION

To study the presence of transforming sequence Bg/II N of HSV-2 in cervical cancer tissues, we developed the nested polymerase chain reaction (PCR) for detecting such a sequence in paraffin-embedded cervical tissue sections. Samples derived from 46 patients with premalignant and malignant lesions were tested. The sequence was found in 20-25% of total cases tested but not observed in any of the normal healthy controls. This study also indicates that for the detection of HSV-2 Bg/II N sequence in cervical tissue, the nested PCR may be more reliable than the in situ hybridization method.

• 出版日期1994-12