Attachment of nanoparticles to pyrolytic graphite electrode and its application for the direct electrochemistry and electrocatalytic behavior of catalase

作者：Zhou Baojuan; Wang Junwei; Gao Xiaoling; Tian Yanni^*

来源：Analytical Letters, 2008, 41(10): 1832-1849.

DOI：10.1080/00032710802161709

• catalase
• electrocatalysis
• GNP
• MWCNT
• hydrogen peroxide

A highly hydrophilic, nontoxic, and conductive effect of colloidal gold nanoparticles (GNP) and multi-walled carbon nanotubes (MWCNT) on pyrolytic graphite electrode has been demonstrated. The direct electron transfer of catalase (CAT) was achieved based on the immobilization of MWCNT/CAT-GNP on a pyrolytic graphite electrode by a Nafion film. The immobilized catalase displayed a pair of well-defined and nearly reversible redox peaks in 0.1M phosphate buffer solution (PBS) (pH 6.98). The dependence of E'on solution pH indicated that the direct electron transfer reaction of catalase was a single-electron-transfer coupled with single-proton-transfer reaction process. The immobilized catalase maintained its biological activity, showing a surface controlled electrode process with an apparent heterogeneous electron transfer rate constant (ks) of 1.3870.1 s-1 and charge-transfer coefficient () of 0.49, and displayed electrocatalytic activity in the electrocatalytic reduction of hydrogen peroxide. Therefore, the resulting modified electrode can be used as a biosensor for detecting hydrogen peroxide.

• 出版日期2008
• 单位山西大学