Development and optimisation of ten novel microsatellite loci in three multiplex PCR panels for the Wolfie Cardinalfish (Cheilodipterus artus)

作者：Underwood Jim N^*

来源：Conservation Genetics Resources, 2010, 2: 261-264.

DOI：10.1007/s12686-010-9182-9

• Apogonidae
• Coral reef fish
• Population connectivity
• Conservation genetics

Primer pairs for ten microsatellite loci of the Wolfie Cardinalfish, Cheilodipterus artus (Apogonidae) were designed from a partial genomic DNA library. The loci were optimised and tested on 48 individuals from a single sampling site. Using three multiplex reactions, each primer pair amplified good quality, reliable and polymorphic product. The number of alleles ranged from 9 to 37 per locus and average expected heterozygosities ranged from 0.780 to 0.964. No evidence of linkage disequilibrium was detected between any pair of loci, while significant departure from Hardy-Weinberg expectation was detected at one locus only, suggesting the presence of a null allele at low frequency at this locus. These markers will be employed to measure genetic connectivity and infer influences of connectivity on population maintenance and resilience in this mouth-brooding coral reef fish that inhabits sheltered lagoons in the coral atolls of north-west of Australia.

• 出版日期2010-9