Toll-like receptor 3 activation promotes desensitization of histamine response in human gingival fibroblasts Poly (I:C) induces histamine receptor desensitization in human gingival fibroblasts

作者：Gutierrez Venegas Gloria^*; Ekaterina Rodriguez Perez Citlalli

来源：Cellular Immunology, 2012, 273(2): 150-157.

DOI：10.1016/j.cellimm.2011.12.005

• Histamine receptor
• Toll like receptor 3
• Protein kinase C
• Receptor desensitization

Viruses are associated with the development of periodontal disease, particularly during periods of suppressed cellular immunity. For this reason, we evaluated the hypothesis that viral components regulate the actions of histamine, an important mediator of immune responses. We assessed the effect of Poly (I:C) on histamine-mediated intracellular calcium mobilization in human gingival fibroblasts. Our results show that histamine induces an increase in intracellular calcium concentrations in a dose-dependent manner. This response was blocked when cells were incubated in the presence of Poly (I:C). In addition, phorbol esters, a diacylglycerol analog, mimics the inhibitory actions of Poly (I:C) in response to histamine. The effect of Poly (I:C) was reversed by Stuarosporine (1 mu M), GO6983 (7 mu M), Bisindolylmaleimide (1 mu M) [a protein inhibitor (PKC)], and SB 203580 (3 mu M) (a p38-MAPK inhibitor). These findings suggest that Poly (I:C) regulates histamine-induced calcium mobilization through activation of PKC and p38.

• 出版日期2012