Nucleation of Hydroxyapatite on Antheraea pernyi (A-pernyi) Silk Fibroin Film

作者：Yang, Mingying^*; Shuai, Yajun; Zhou, Guanshan; Mandal, Namita; Zhu, Liangjun

来源：Bio-medical Materials and Engineering, 2014, 24(1): 731-740.

DOI：10.3233/BME-130861

• Antheraea pernyi fibroin
• hydroxyapatite
• mineralization
• cell culture

Antheraea pernyi (A. pernyi) silk fibroin, which is spun from a wild silkworm, has increasingly attracted interest in the field of tissue engineering. The aim of this study was to investigate the nucleation of hydroxyapatite (HAp) on A. pernyi fibroin film. Von Kossa staining proved that A. pernyi fibroin had Ca binding activity. The A. pernyi fibroin film was mineralized with HAp crystals by alternative soaking in calcium and phosphate solutions. Spherical crystals were nucleated on the A. pernyi fibroin film according to scanning electron microscope imaging results. The FT-IR and X-ray diffraction spectra confirmed that these spherical crystals were HAp. The results of in vitro cell culture using MG-63 cells demonstrated that the mineralized A. pernyi fibroin film showed excellent cytocompatibility and sound improvement of the MG-63 cell viability.

• 出版日期2014
• 单位浙江大学