Micro RNA-19a suppresses IL-10 in peripheral B cells from patients with atherosclerosis

作者：Ren, Zhong-Qiao; Liu, Ning; Zhao, Kan^*

来源：Cytokine, 2016, 86: 86-91.

DOI：10.1016/j.cyto.2016.07.019

• Interleukin-10
• Lymphocyte
• Atherosclerosis
• Micro RNA
• p300

Background and aims: The interleukin (IL)-10-production B cells play an important role in the pathogenesis of atherosclerosis (Asro) with unknown mechanism. Micro RNA (miR)-17-92 cluster has strong immune regulatory activities. This study tests a hypothesis that miR-17-92 cluster suppresses IL-10 expression in B cells of Asro patients. @@@ Methods: Patients with Asro were recruited into this study. Peripheral blood samples were collected from the patients. B cells were isolated from the blood samples and analyzed to elucidate the role of miR-17-92 in the regulation of IL-10 expression. @@@ Results: Peripheral B cells from patients with Asro show lower levels of IL-10 than that from healthy subjects. The IL-10 expression in the B cells is negatively correlated with the expression of miR-19a in the B cells. The serum levels of tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma and interleukin (IL)-4 in Asro patients were higher than healthy subjects. Exposure to TNF-alpha or IFN-gamma or IL-4 suppressed IL-10 expression in B cells via increasing the expression of miR-19a in B cells, which could be abolished by Inhibition of miR-19a. @@@ Conclusions: TNF-alpha, or IFN-gamma or IL-4 suppresses IL-10 in B cells via up regulating miR-19a expression.

• 出版日期2016-10
• 单位哈尔滨医科大学