High-fat diet during pregnancy and lactation impairs the cholinergic anti-inflammatory pathway in the liver and white adipose tissue of mouse offspring

作者：Payolla Tanyara Baliani; Lemes Simone Ferreira; de Fante Thais; Reginato Andressa; da Silva Cristiano Mendes; Micheletti Thayana de Oliveira; Rodrigues Hosana Gomes; Torsoni Adriana Souza; Milanski Marciane; Torsoni Marcio Alberto^*

来源：Molecular and Cellular Endocrinology, 2016, 422(C): 192-202.

DOI：10.1016/j.mce.2015.12.004

• alpha 7nAChR
• Cholinergic anti-inflammatory pathway
• LPS
• Maternal imprinting
• Obese mice
• High-fat diet

Cholinergic anti-inflammatory pathway (CAP) prevents inflammatory cytokines production. The main was to evaluate the effect of maternal obesity on cholinergic pathway in the offspring. Female mice were subjected to either standard chow (SC) or high-fat diet (HFD) during pregnancy and the lactation period. After weaning, only male offspring from HFD dams (HFD-O) and from SC dams (SC-O) were fed the SC diet. Key proteins of the CAP were downregulated and serum TNF-alpha was elevated in the HFD-O mice. STAT3 and NF-kappa B activation in HFD-O mice ICV injected with nicotine (agonist) were lower than SC-O mice. Basal cholinesterase activity was upregulated in HFD-O mice in both investigated tissues. Lipopolysaccharide increased TNF-alpha and IL-1 beta expression in the liver and WAT of SC-O mice, but this effect was greater in HFD-O mice. In conclusion these changes exacerbated cytokine production in response to LPS and contributed to the reduced sensitivity of the CAP.

• 出版日期2016-2-15