科研之友
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摘要
Guanine-rich nucleic acid sequence can fold into guanine quadruplex (G-quadruplex) that is found to complex tightly with hemin to form the hemin/G-quadruplex. Under physiological conditions, the hemin/G-quadruplex displays robust peroxidase activity and acts as horseradish peroxidase (HRP)-mimicking DNAzyme. As a kind of artificial enzyme, the hemin/G-quadruplex DNAzyme is much more stable against hydrolysis and heat treatment than natural enzymes and can be synthesized in the laboratory and modified easily. Thus, it has been considered as a promising tool for the design of sensors. Electrochemical sensor is one of the major analytical techniques for sensitive and selective detection of proteins, DNA as well as small biomolecules. In this work, we reviewed the strategies for designing of electrochemical sensors based on the signal amplification of hemin/G-quadruplex DNAzyme.
- 出版日期2015-5
- 单位桂林电子科技大学
