Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours

作者：Abou Asa S; Mori T; Maruo K; Khater A; El sawak A; el Aziz E Abd; Yanai T; Sakai H^*

来源：Veterinary and Comparative Oncology, 2015, 13(3): 237-245.

DOI：10.1111/vco.12035

• dog
• hemangioma
• hemangiosarcoma
• immunohistochemistry
• mutation
• PDGFR

We examined whether mutation of the platelet-derived growth factor receptor protein tyrosine kinase (PDGFR)- and PDGFR- genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans- or juxtamembrane regions of PDGFR- and PDGFR- were analysed with immunohistochemical staining and polymerase chain reaction-direct sequencing using DNA from paraffin-embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR- and almost most of the HA cases were negative for PDGFR-. Of the HSA cases, 55.6% were negative for PDGFR- and 63% were strongly positive for PDGFR-. Among the HA cases, 1 missense mutation was detected in PDGFR- exon 18 and 1 in PDGFR- exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR-. Thus, genomic mutation of trans- or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA.

• 出版日期2015-9