Altered Th17 Cytokine Expression in Helicobacter pylori Patients with TLR4 (D299G) Polymorphism

作者：Bagheri Nader; Azadegan Dehkordi Fatemeh; Rahimian Ghorb****i; Hashemzadeh Chaleshtori Morteza; Rafieian Kopaei Mahmoud; Kheiri Soleyman; Gholipour Abolfazl; Shirzad Hedayatollah

来源：Immunological Investigations, 2016, 45(2): 161-171.

DOI：10.3109/08820139.2015.1122615

• TLR4
• Cytokine
• Th17
• Helicobacter pylori
• gene polymorphism
• gastritis

Helicobacter pylori (H. pylori) is associated with gastric ulcer and gastric adenocarcinoma. Polymorphisms in the host genes coding for Toll-like receptors (TLRs) may influence the innate and adaptive immune response to the infection, affecting the susceptibility to H. pylori or the disease outcomes. However, the details and association with different polymorphism and clinical expression of infection remain unclear. A case-control study consisting of 58 patients with H. pylori infection and 44 H. pylori uninfection was conducted. Genomic DNA was extracted and genotypes of TLR4 Asp299Gly polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Mucosal cytokines expression in H. pylori-infected and uninfected gastric biopsies was determined by real-time PCR. The expression of IL-6, IL-17, IL-21, IL-23 and TGF-beta 1 was signi?cantly higher in patients with D299G polymorphism in TLR4. But the expression of IL-18 between patients with single-nucleotide polymorphisms (SNPs) in TLR4 and patients with the wild-type allele was not signi?cant. In H. pylori-infected patients with gastritis, SNPs in TLR4 may alter cytokine expression toward Th17 immune response in the gastric mucosa and may have increased risk for the development of peptic ulcer.

• 出版日期2016-2-17