Botulinum Toxin Complex Increases Paracellular Permeability in Intestinal Epithelial Cells via Activation of p38 Mitogen-Activated Protein Kinase

作者：Miyashita Shin ichiro; Sagane Yoshimasa; Inui Ken; Hayashi Shintaro; Miyata Keita; Suzuki Tomonori; Ohyama Tohru; Watanabe Toshihiro; Niwa Koichi^*

来源：Journal of Veterinary Medical Science, 2013, 75(12): 1637-1642.

DOI：10.1292/jvms.13-0164

• botulinum toxin complex
• Clostridium botulinum
• intestinal epithelial cells
• mitogen-activated protein kinase

Clostridium botulinum produces a large toxin complex (L-TC) that increases paracellular permeability in intestinal epithelial cells by a mechanism that remains unclear. Here, we show that mitogen-activated protein kinases (MAPKs) are involved in this permeability increase. Paracellular permeability was measured by FITC-dextran flux through a monolayer of rat intestinal epithelial IEC-6 cells, and MAPK activation was estimated from western blots. L-TC of C. botulinum serotype D strain 4947 increased paracellular dextran flux and activated extracellular signal-regulated kinase (ERK), p38, but not c-Jun N-terminal kinase (JNK) in IEC-6 cells. The permeability increase induced by L-TC was abrogated by the p38 inhibitor SB203580. These results indicate that L-TC increases paracellular permeability by activating p38, but not INK and ERK.

• 出版日期2013-12